Dr. Liu Zhemin | Protein Engineering | Best Researcher Award
Senior Scientist at Nanjing Vazyme Biotechnology Company, China
Publication Profile :Β
Educational Background π
Z. Liu has a strong academic foundation in biochemistry and molecular biology, with advanced training in enzyme engineering and microbial biotechnology. His education laid the groundwork for his expertise in protein design, directed evolution, and enzyme applications.
Professional Experience πΌ
- Z. Liu, X. Fu, M. Yuan, Q. Liang, C. Zhu, H. Mou, Surface charged amino acid-based strategy for rational engineering of kinetic stability and specific activity of enzymes: Linking experiments with computational modeling. Int. J. Biol. Macromol. 182, 228β236 (2021).
- M. Wu, L. Cao, W. Tang, Z. Liu*, S. Feng, Improving the anti-autolytic ability of alkaline protease from Bacillus alcalophilus by a rationally combined strategy. Enzyme Microb. Technol., 110561 (2024).
- Z. Liu, L. Cao, X. Fu, Q. Liang, H. Sun, H. Mou, *A multi-functional genetic manipulation system and its use in high-level expression of a Ξ²-mannanase mutant with high specific activity in Pichia pastoris. Microb. Biotechnol. 14, 1525β1538 (2021).
- W. Zhang#, Z. Liu#, S. Zhou, H. Mou, R. Zhang, Cloning and expression of a Ξ²-mannanase gene from Bacillus sp. MK-2 and its directed evolution by random mutagenesis. Enzyme Microb. Technol. 124, 70β78 (2019).
- Z. Liu, C. Ning, M. Yuan, X. Fu, S. Yang, X. Wei, M. Xiao, H. Mou, C. Zhu, High-efficiency expression of a superior Ξ²-mannanase engineered by cooperative substitution method in Pichia pastoris and its application in preparation of prebiotic mannooligosaccharides. Bioresour. Technol. 311, 123482 (2020).
- Z. Liu, L. Cao, X. Fu, Q. Liang, H. Sun, H. Mou, *A multi-functional genetic manipulation system and its use in high-level expression of a Ξ²-mannanase mutant with high specific activity in Pichia pastoris. Microb. Biotechnol. 14, 1525β1538 (2021).
- Q. Liang, L. Cao, C. Zhu, Q. Kong, H. Sun, F. Zhang, H. Mou, Z. Liu*, *Characterization of recombinant antimicrobial peptide BMGlv2 heterologously expressed in Trichoderma reesei. Int. J. Mol. Sci. 23 (2022). doi:10.3390/ijms231810291.
- W. Zhang#, Z. Liu#, S. Zhou, H. Mou, R. Zhang, Cloning and expression of a Ξ²-mannanase gene from Bacillus sp. MK-2 and its directed evolution by random mutagenesis. Enzyme Microb. Technol. 124, 70β78 (2019).
- Z. Liu, C. Ning, M. Yuan, X. Fu, S. Yang, X. Wei, M. Xiao, H. Mou, C. Zhu, High-efficiency expression of a superior Ξ²-mannanase engineered by cooperative substitution method in Pichia pastoris and its application in preparation of prebiotic mannooligosaccharides. Bioresour. Technol. 311, 123482 (2020).
- Z. Liu, C. Ning, M. Yuan, S. Yang, X. Wei, M. Xiao, X. Fu, C. Zhu, H. Mou, High-level expression of a thermophilic and acidophilic beta-mannanase from Aspergillus kawachii IFO 4308 with significant potential in mannooligosaccharide preparation. Bioresour. Technol. 295, 122257 (2019).